Introduction of the harpinXooc-encoding gene hrf2 in soybean enhances resistance against the oomycete pathogen Phytophthora sojae.
Identifieur interne : 000469 ( Main/Exploration ); précédent : 000468; suivant : 000470Introduction of the harpinXooc-encoding gene hrf2 in soybean enhances resistance against the oomycete pathogen Phytophthora sojae.
Auteurs : Lu Niu [République populaire de Chine] ; Jing Yang [République populaire de Chine] ; Jinhua Zhang [République populaire de Chine] ; Hongli He [République populaire de Chine] ; Guojie Xing [République populaire de Chine] ; Qianqian Zhao [République populaire de Chine] ; Dongquan Guo [République populaire de Chine] ; Li Sui [République populaire de Chine] ; Xiaofang Zhong [République populaire de Chine] ; Xiangdong Yang [République populaire de Chine]Source :
- Transgenic research [ 1573-9368 ] ; 2019.
Descripteurs français
- KwdFr :
- Interactions hôte-parasite (génétique), Maladies des plantes (génétique), Maladies des plantes (parasitologie), Phytophthora (pathogénicité), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Résistance à la maladie (MeSH), Soja (croissance et développement), Soja (génétique), Soja (parasitologie), Transduction du signal (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (parasitologie).
- MESH :
- croissance et développement : Soja, Végétaux génétiquement modifiés.
- génétique : Interactions hôte-parasite, Maladies des plantes, Protéines végétales, Soja, Végétaux génétiquement modifiés.
- métabolisme : Protéines végétales.
- parasitologie : Maladies des plantes, Soja, Végétaux génétiquement modifiés.
- pathogénicité : Phytophthora.
- Régulation de l'expression des gènes végétaux, Résistance à la maladie, Transduction du signal.
English descriptors
- KwdEn :
- Disease Resistance (MeSH), Gene Expression Regulation, Plant (MeSH), Host-Parasite Interactions (genetics), Phytophthora (pathogenicity), Plant Diseases (genetics), Plant Diseases (parasitology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (parasitology), Signal Transduction (MeSH), Soybeans (genetics), Soybeans (growth & development), Soybeans (parasitology).
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Host-Parasite Interactions, Plant Diseases, Plants, Genetically Modified, Soybeans.
- growth & development : Plants, Genetically Modified, Soybeans.
- chemical , metabolism : Plant Proteins.
- parasitology : Plant Diseases, Plants, Genetically Modified, Soybeans.
- pathogenicity : Phytophthora.
- Disease Resistance, Gene Expression Regulation, Plant, Signal Transduction.
Abstract
Phytophthora root and stem rot (PRR) caused by an oomycete pathogen Phytophthora sojae is one of the most devastating and widespread diseases throughout soybean-producing regions worldwide. The diversity and variability of P. sojae races make effective control of the pathogen challenging. Here, we introduced an elicitor of plant defense response, the harpinXooc-encoding hrf2 gene from the rice bacterial pathogen Xanthomonas oryzae pv. oryzicola into soybean and evaluated resistance to P. sojae infection. Molecular analysis confirmed the integration and expression of hrf2 in the transgenic soybean. After inoculation with P. sojae, non-transformed control (NC) plants exhibited typical PRR symptoms, including necrotic and wilting leaves, and plant death, whereas most of the transgenic plants showed slightly chlorotic leaves and developed normally. Through T3 to T5 generations, the transgenic events displayed milder disease symptoms and had higher survival rates compared to NC plants, indicating enhanced and stable resistance to P. sojae infection, whereas without P. sojae inoculation, no significant differences in agronomic traits were observed between the transgenic and non-transformed plants. Moreover, after inoculation with P. sojae, significant upregulation of a set of plant defense-related genes, including salicylic acid- and jasmonic acid-dependent and hypersensitive response-related genes was observed in the transgenic plants. Our results indicate that hrf2 expression in transgenic soybean significantly enhanced resistance to P. sojae by eliciting multiple defense responses mediated by different signaling pathways. The potential functional role of the hrf2 gene in plant defense against P. sojae and other pathogens makes it a promising tool for broadening disease resistance in soybean.
DOI: 10.1007/s11248-019-00119-4
PubMed: 30830582
Affiliations:
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uniqKey="Niu L" first="Lu" last="Niu">Lu Niu</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Jing" sort="Yang, Jing" uniqKey="Yang J" first="Jing" last="Yang">Jing Yang</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Jinhua" sort="Zhang, Jinhua" uniqKey="Zhang J" first="Jinhua" last="Zhang">Jinhua Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="He, Hongli" sort="He, Hongli" uniqKey="He H" first="Hongli" last="He">Hongli He</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Xing, Guojie" sort="Xing, Guojie" uniqKey="Xing G" first="Guojie" last="Xing">Guojie Xing</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Qianqian" sort="Zhao, Qianqian" uniqKey="Zhao Q" first="Qianqian" last="Zhao">Qianqian Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Dongquan" sort="Guo, Dongquan" uniqKey="Guo D" first="Dongquan" last="Guo">Dongquan Guo</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Sui, Li" sort="Sui, Li" uniqKey="Sui L" first="Li" last="Sui">Li Sui</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Zhong, Xiaofang" sort="Zhong, Xiaofang" uniqKey="Zhong X" first="Xiaofang" last="Zhong">Xiaofang Zhong</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiangdong" sort="Yang, Xiangdong" uniqKey="Yang X" first="Xiangdong" last="Yang">Xiangdong Yang</name><affiliation wicri:level="1"><nlm:affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China. xdyang020918@126.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033</wicri:regionArea><wicri:noRegion>130033</wicri:noRegion></affiliation></author></analytic><series><title level="j">Transgenic research</title><idno type="eISSN">1573-9368</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Resistance (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Host-Parasite Interactions (genetics)</term><term>Phytophthora (pathogenicity)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (parasitology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (parasitology)</term><term>Signal Transduction (MeSH)</term><term>Soybeans (genetics)</term><term>Soybeans (growth & development)</term><term>Soybeans (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Interactions hôte-parasite (génétique)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (parasitologie)</term><term>Phytophthora (pathogénicité)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Résistance à la maladie (MeSH)</term><term>Soja (croissance et développement)</term><term>Soja (génétique)</term><term>Soja (parasitologie)</term><term>Transduction du signal (MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (parasitologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Soja</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Host-Parasite Interactions</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plants, Genetically Modified</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Interactions hôte-parasite</term><term>Maladies des plantes</term><term>Protéines végétales</term><term>Soja</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term><term>Soja</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Resistance</term><term>Gene Expression Regulation, Plant</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term><term>Résistance à la maladie</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytophthora root and stem rot (PRR) caused by an oomycete pathogen Phytophthora sojae is one of the most devastating and widespread diseases throughout soybean-producing regions worldwide. The diversity and variability of P. sojae races make effective control of the pathogen challenging. Here, we introduced an elicitor of plant defense response, the harpin<sub>Xooc</sub>-encoding hrf2 gene from the rice bacterial pathogen Xanthomonas oryzae pv. oryzicola into soybean and evaluated resistance to P. sojae infection. Molecular analysis confirmed the integration and expression of hrf2 in the transgenic soybean. After inoculation with P. sojae, non-transformed control (NC) plants exhibited typical PRR symptoms, including necrotic and wilting leaves, and plant death, whereas most of the transgenic plants showed slightly chlorotic leaves and developed normally. Through T<sub>3</sub> to T<sub>5</sub> generations, the transgenic events displayed milder disease symptoms and had higher survival rates compared to NC plants, indicating enhanced and stable resistance to P. sojae infection, whereas without P. sojae inoculation, no significant differences in agronomic traits were observed between the transgenic and non-transformed plants. Moreover, after inoculation with P. sojae, significant upregulation of a set of plant defense-related genes, including salicylic acid- and jasmonic acid-dependent and hypersensitive response-related genes was observed in the transgenic plants. Our results indicate that hrf2 expression in transgenic soybean significantly enhanced resistance to P. sojae by eliciting multiple defense responses mediated by different signaling pathways. The potential functional role of the hrf2 gene in plant defense against P. sojae and other pathogens makes it a promising tool for broadening disease resistance in soybean.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30830582</PMID><DateCompleted><Year>2019</Year><Month>08</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-9368</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>2</Issue><PubDate><Year>2019</Year><Month>04</Month></PubDate></JournalIssue><Title>Transgenic research</Title><ISOAbbreviation>Transgenic Res</ISOAbbreviation></Journal><ArticleTitle>Introduction of the harpin<sub>Xooc</sub>-encoding gene hrf2 in soybean enhances resistance against the oomycete pathogen Phytophthora sojae.</ArticleTitle><Pagination><MedlinePgn>257-266</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11248-019-00119-4</ELocationID><Abstract><AbstractText>Phytophthora root and stem rot (PRR) caused by an oomycete pathogen Phytophthora sojae is one of the most devastating and widespread diseases throughout soybean-producing regions worldwide. The diversity and variability of P. sojae races make effective control of the pathogen challenging. Here, we introduced an elicitor of plant defense response, the harpin<sub>Xooc</sub>-encoding hrf2 gene from the rice bacterial pathogen Xanthomonas oryzae pv. oryzicola into soybean and evaluated resistance to P. sojae infection. Molecular analysis confirmed the integration and expression of hrf2 in the transgenic soybean. After inoculation with P. sojae, non-transformed control (NC) plants exhibited typical PRR symptoms, including necrotic and wilting leaves, and plant death, whereas most of the transgenic plants showed slightly chlorotic leaves and developed normally. Through T<sub>3</sub> to T<sub>5</sub> generations, the transgenic events displayed milder disease symptoms and had higher survival rates compared to NC plants, indicating enhanced and stable resistance to P. sojae infection, whereas without P. sojae inoculation, no significant differences in agronomic traits were observed between the transgenic and non-transformed plants. Moreover, after inoculation with P. sojae, significant upregulation of a set of plant defense-related genes, including salicylic acid- and jasmonic acid-dependent and hypersensitive response-related genes was observed in the transgenic plants. Our results indicate that hrf2 expression in transgenic soybean significantly enhanced resistance to P. sojae by eliciting multiple defense responses mediated by different signaling pathways. The potential functional role of the hrf2 gene in plant defense against P. sojae and other pathogens makes it a promising tool for broadening disease resistance in soybean.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Niu</LastName><ForeName>Lu</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jinhua</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Hongli</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xing</LastName><ForeName>Guojie</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Qianqian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Dongquan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sui</LastName><ForeName>Li</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhong</LastName><ForeName>Xiaofang</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiangdong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China. xdyang020918@126.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>03</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Transgenic Res</MedlineTA><NlmUniqueID>9209120</NlmUniqueID><ISSNLinking>0962-8819</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><MeshHeadingList><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="Y">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006790" MajorTopicYN="N">Host-Parasite Interactions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant 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Owner="NOTNLM"><Keyword MajorTopicYN="Y">Defense response</Keyword><Keyword MajorTopicYN="Y">Harpin</Keyword><Keyword MajorTopicYN="Y">Phytophthora sojae</Keyword><Keyword MajorTopicYN="Y">Phytophthora stem and root rot</Keyword><Keyword MajorTopicYN="Y">Soybean</Keyword><Keyword MajorTopicYN="Y">hrf2</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>12</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>02</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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sortKey="Xing, Guojie" sort="Xing, Guojie" uniqKey="Xing G" first="Guojie" last="Xing">Guojie Xing</name><name sortKey="Yang, Jing" sort="Yang, Jing" uniqKey="Yang J" first="Jing" last="Yang">Jing Yang</name><name sortKey="Yang, Xiangdong" sort="Yang, Xiangdong" uniqKey="Yang X" first="Xiangdong" last="Yang">Xiangdong Yang</name><name sortKey="Zhang, Jinhua" sort="Zhang, Jinhua" uniqKey="Zhang J" first="Jinhua" last="Zhang">Jinhua Zhang</name><name sortKey="Zhao, Qianqian" sort="Zhao, Qianqian" uniqKey="Zhao Q" first="Qianqian" last="Zhao">Qianqian Zhao</name><name sortKey="Zhong, Xiaofang" sort="Zhong, Xiaofang" uniqKey="Zhong X" first="Xiaofang" last="Zhong">Xiaofang Zhong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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